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圈养华北豹粪便样品中雌二醇提取方法的研究
Authors
李亮亮 1 由玉岩 2 金修哲 1 张卓 1 孙乐天 1 卫泽珍 1 杨引亮 1 刘学锋 2 张成林 2 李晓光 2
Publication date
1 January 2020
Publisher
Editorial Department of Chinese Journal of Wildlife
Abstract
本研究以圈养华北豹粪便样品中雌二醇的提取方法为研究对象。本实验中比较了甲醇加热法、乙醇加热法和乙醇蒸馏水,3种粪样中提取雌二醇的技术方法,利用放射免疫分析法测定雌二醇的含量。结果显示,甲醇加热法、乙醇加热法和乙醇蒸馏水法3种方法分别提取浓度为(281547±59747)pg/mL、(766025±154611)pg/mL和(193783±53171)pg/mL。其中乙醇加热法是这3种方法中提取含量可达(766025±154611)pg/mL,通过统计学分析发现,乙醇加热法与其他2种方法差异显著。实验证明:利用乙醇加热法提取圈养华北豹粪便雌二醇是一种比较理想的方法
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Last time updated on 03/04/2025