https://digitalcommons.library.uab.edu/sp-expo/1009/thumbnail.jp

Dong, Lilly

Pierini, Filippo

Stanishevsky, Andrei

Zakrzewska, Anna

English

2024 Fall Expo Poster Presentationhttps://digitalcommons.library.uab.edu/sp-expo/1009/thumbnail.jp

UAB Digital Commons (Univ. of Alabama at Birmingham)

kvOptimization of Spinnability of Stimuli-Responsive Nanofibers in Alternating Current ElectrospinningLilly Dong, Andrei Stanishevsky, Filippo Pierini, and Anna ZakrzewskaElectrospinning, lPoly(N, N-dimethylacrylamide) FibersTypically, direct current electrospinning (DCES) is used to synthesize nanofibers, but alternating current electrospinning (ACES) was explored because of its possibility for cheaper and more efficient production.Here, a multi-layered system of stimuli-responsive nanofibrous materials is being pursued for on-demand drug delivery. Combining an infrared-responsive layer composed of poly(N, N-dimethyl acrylamide)(PMA) with evenly distributed superparamagnetic iron-oxide nanoparticles (SPIONs) and a thermo-responsive layer of poly(n-isopropyl acrylamide) (PiPAM) - cold fish gelatin (CFG) composite could present withsuccess.There is a large research gap of successful ACES precursor solutions, so many recipes have been modelled after those recorded in DCES literature. Much of this project’s early focus is on the successful synthesis ofnanofibers and their following characterization with SEM, FTIR, and mechanical testing.(Left) General set up for DCES [2].PMA has been used in nanofibroushydrogels because of its self-healingproperties and high levels of water retentionwhich presents potential for drug loadingcapabilities [3]. PMA was successfully spunusing CFG as a carrier. CFG demonstratesgood biocompatibility, ethical production,potential for drug loading, and well-knownmethods of cross-linking [4].DHTNoYesBAYesGTADCNo(Above) Cross-linking methods whether dehydrothermal (DHT) treatment or exposure to glutaraldehyde vapors (GTA).00.51Stress (MPa)Cross-linking MethodsMaximum Stress Endured of Cross-linked PMA/CFGDCBAPiPAM is a thermo-responsivepolymer that has been spun in DCES[5]. Currently testing possible PiPAMsolutions in ACES.References Conclusion Stimuli-ResponseMulti-Layered SystemSPIONs have shownelectromagnetic heatingproperties:• Exposure to an ACmagnetic field [6]• Irradiation of near-infrared (NIR) light [6]Electrospraying ontofibers can evenlydistribute SPIONs.• Drug-loading layer: PMA/CFGfibers• Electromagnetic-responsive layer:Nanofibers with evenly distributedSPIONs• Thermo-responsive layer: PiPAMfibersCascade of Stimuli-responsesExposure to AC magnetic field orNIR light causes SPIONs to generateheat, causing PiPAM to overcome itsLCT. PiPAM becomes hydrophobic,causing a release of water and drugsfrom PMA/CFG.Synthesis of PMA/CFG fibers provedsuccessful. Continued work to synthesizePiPAM fibers and incorporate SPIONs iscrucial before studying the proposed multi-layered drug delivery system.Further study will include optimization offibers, confirmation of stimuli-response, andcharacterization as a multi-layered system.AcknowledgementPrimary author and this study have beensupported by the NSF International ResearchExperience for Students (IRES) award to UAB(Grant Number OISE-1852207). Thank you to IPPTPAN for use of their facilities.[1] Valtera, J., et al. (2019). Fabrication of dual-functional composite yarns with a nanofibrous envelope using high throughput AC needleless and collectorless electrospinning. Scientific Reports, 9(1). [2] Elyor Berdimurodov, et al. (2023). Green Electrospun Nanofibers for Biomedicine and Biotechnology. Technologies (Basel), 11(5), 150–150. [3] Du, W., et al. (2019). An injectable self-healing hydrogel-cellulose nanocrystals conjugate with excellent mechanical strength and good biocompatibility. Carbohydrate Polymers, 223, 115084. [4] Rathna, G. V. N. (2007). Gelatin hydrogels: enhanced biocompatibility, drug release and cell viability. Journal of Materials Science: Materials in Medicine, 19(6), 2351–2358. [5] Farahat, D. S., et al. (2024). Poly(N‐isopropylacrylamide) based smart nanofibrous scaffolds for use as on‐demand delivery systems for oral and dental tissue regeneration. Journal of Biomedical Materials Research Part A, 112(6), 852–865. [6] Vangijzegem, T., et al. (2023). Superparamagnetic Iron Oxide Nanoparticles (SPION): From Fundamentals to State-of-the-Art Innovative Applications for Cancer Therapy. Pharmaceutics, 15(1), 236–236.  (Right) Diagram displaying PiPAM’s thermo-responsiveness. PiPAM’s relationship with water can  fluctuate based on its lower critical temperature (LCT)-100102030400 0.5Number of FibersFiber Diameter (µm)Distribution of Fiber Diameter of PMA/FG Cross-linking MethodsDCBA(Right) Fiber diameter distribution analyzed using ImageJ software.(Left) Strength of fibers measured by maximum strength endured which was obtained through mechanical testing.(Right) Possible chemical changes caused by cross-linking methods analyzed using FTIR.(Left) SEM imaging of fibers with correspondinglabels from crosslinking table. Fibers thatbecame insoluble in water were dried and SEMwas captured, labelled wet.DCB B:wetA:wetAHydrophobic32℃ <Hydrophilic32℃ >(Above left) Diagram showing alternating charges created by ACES.(Above right) Picture of fibrous plume created by ACES [1].Superparamagnetic Iron-Oxide NanoparticlesPoly(n-isopropylacrylamide) Fibers

Optimization of Spinnability of Stimuli-Responsive Nanofibers in Alternating Current Electrospinning

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Optimization of Spinnability of Stimuli-Responsive Nanofibers in Alternating Current Electrospinning

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