Due to the increasing use of nanometric cerium oxide in applications,
concerns about the toxicity of these particles have been raised and have
resulted in a large number of investigations. We report here on the
interactions between 7 nm anionically charged cerium oxide particles and living
mammalian cells. By a modification of the particle coating including
low-molecular weight ligands and polymers, two generic behaviors are compared:
particles coated with citrate ions that precipitate in biofluids and particles
coated with poly(acrylic acid) that are stable and remain nanometric. We find
that nanoceria covered with both coating agents are taken up by mouse
fibroblasts and localized into membrane-bound compartments. However, flow
cytometry and electron microscopy reveal that as a result of their
precipitation, citrate-coated particles interact more strongly with cells. At
cerium concentration above 1 mM, only citrate-coated nanoceria (and not
particles coated with poly(acrylic acid)) display toxicity and moderate
genotoxicity. The results demonstrate that the control of the surface chemistry
of the particles and its ability to prevent aggregation can affect the toxicity
of nanomaterials.Comment: 33 pages 10 figures, accepted at Nanotoxicolog