INABA Kazuo

Nakajima Ayako

Nomura Mamoru

Developmental Biology

Tsukuba Repository  (Tulips-R)

Proteomic profiles of embryonic development inthe ascidian Ciona intestinalis著者 Nomura Mamoru, Nakajima Ayako, Inaba Kazuojournal orpublication titleDevelopmental biologyvolume 325number 2page range 468-481year 2009-01権利 (C) 2008 Elsevier IncURL http://hdl.handle.net/2241/101753doi: 10.1016/j.ydbio.2008.10.038rTable 1. Attribution of proteins identified in unfertilized eggs, 16 cell-stage embryos and tadpole larvae to the gene clusters from microarraygene expression profile.Category*1Percentage (Protein count)Total Unfertilized eggs 16 cell-stage embryos Larvaea Embryonic gene cluster 7 (16) 6 (3) 0 (0) 10 (13)b Embryonic and adult gene cluste 16 (36) 9 (5) 15 (6) 20 (25)c Adult gene cluster 17 (38) 20 (11) 20 (8) 15 (19)d Stably expressed gene cluster 10 (22) 6 (3) 7 (3) 13 (16)e Maternal gene cluster 49 (109) 59 (32) 59 (24) 42 (53)*1 Category of gene cluster is according to Azumi et al., 2007.Table 2. List of proteins abundantly expressed in unfertilized eggs, 16 cell-stage embryos and tadpole larvae.SSN Mr(kDa) QTY C.V. Sequence ID Description E-valueUnfertilizedeggs( / 29444)Cleavingembryos( / 26796)Tadpolelarvae( / 24532)ClusterUnfertilized eggs1 A7109 34.36 833.70 0.21 Not determined2 B9202 37.17 833.58 1.28 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164563 A6111 34.44 553.60 0.13 Not determined4 A8008 28.98 509.05 0.29 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164565 A6012 30.78 487.48 0.44 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164566 B8401 44.94 431.69 0.43 KYOTOGRAIL2005.55.42.3 elongation factor 1A [Geodia cydonium] 1.0E-37 103 189 187 05079, 14095, 13525,00080, 103217 B7001 24.70 397.41 1.06 KYOTOGRAIL2005.216.2.1 H+ transporting ATP synthase O subunit isoform 1 [Bombyx 1.0E-37 19 16 3 02586, 017368 B9201 37.08 389.14 1.02 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164569 B7002 26.93 374.86 1.04 KYOTOGRAIL2005.119.5.1 novel protein (zgc:56136) [Danio rerio] 6.0E-28 20 6 10 36808, 0028610 A2209 45.10 349.66 0.12 PROCITS_00805_1_2_0 actin [Ciona intestinalis] 0.0E+00 0 0 0 0004611 B4204 38.12 310.17 0.18 JGI_216024 isocitrate dehydrogenase 3, beta subunit [Mus musculus] 3.0E-122 20 6 0 0424812 A7302 50.50 299.15 0.20 JGI_298739 mitochondrial citrate synthase precursor [Xiphias gladius] 0.0E+00 38 3 2 02113, 0080913 B4504 60.69 285.74 1.24 KYOTOGRAIL2005.100.2.1 No similarity (Vitellogenin) 0 0 0 0099714 B5405 49.24 269.02 0.27 JGI_292814 ATP synthase alpha-subunit [Ciona intestinalis] (mitochondrialF1 complex) 0.0E+00 33 8 4 0039615 A5808 105.64 268.27 0.14 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645616 A4107 37.55 254.99 0.14 KYOTOGRAIL2005.26.37.1 malate dehydrogenase, mitochondrial [Rattus norvegicus] 3.0E-127 18 9 1 0466817 A6813 106.34 205.01 0.13 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645618 B9107 34.81 201.88 0.32 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 16456JGI_233126 * No similarity 34 22 1 00034JGI_224382 * ATP synthase, H+ transporting, mitochondrial F0 complex,subunit b, isoform 1 [Danio rerio] 3.0E-34 34 22 1 0003420 A5812 104.67 196.95 0.08 KYOTOGRAIL2005.100.11.1 No similarity (Vitellogenin) NA NA NA NA21 A8004 28.55 191.93 0.18 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645622 A7206 41.98 190.54 0.33 JGI_294238 isocitrate dehydrogenase 3 (NAD+) alpha [Mus musculus] 4.0E-158 25 9 0 0442223 A5314 48.77 169.22 0.29 KYOTOGRAIL2005.45.49.1 phosphoglycerate kinase [Halocynthia roretzi] 1.0E-171 7 2 1 0567624 B7105 29.18 168.16 0.55 KYOTOGRAIL2005.100.2.1 No similarity (Vitellogenin) 0 0 0 0099725 B7101 31.46 167.36 0.79 KYOTOGRAIL2005.545.3.1 Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2) 7.0E-89 38 61 4 00121, 3661326 B7004 26.95 166.79 1.17 Not determined27 B4107 34.99 160.02 0.35 PROCITS_00881_0_1_0 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 2.0E-154 49 54 19 00881, 37267, 3613928 A5012 31.00 156.89 0.36 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645629 A7007 28.77 148.48 0.65 KYOTOGRAIL2005.10.70.1 No similarity 3 9 3 0018830 A9501 65.30 147.24 0.21 KYOTOGRAIL2005.42.4.1 t-complex polypeptide 1 [Danio rerio] 0.0E+00 4 1 0 12274Blast EST count19 A1005 29.57 197.76 0.19Table 2. Continued1 B8401 44.94 1051.94 0.76 KYOTOGRAIL2005.55.42.3 translation elongation factor 1A 8.0E-170 103 189 187 05079, 14095, 13525,00080, 103212 A6111 34.44 642.37 0.46 Not determined3 B7001 24.70 592.97 0.28 KYOTOGRAIL2005.216.2.1 H+ transporting ATP synthase O subunit isoform 1 [Bombyx 1.0E-37 19 16 3 02586, 017364 B9202 37.17 494.48 2.06 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164565 A2209 45.10 398.13 0.36 PROCITS_00805_1_2_0 actin [Ciona intestinalis] 0.0E+00 0 0 0 000466 B7002 26.93 395.50 0.92 KYOTOGRAIL2005.119.5.1 novel protein (zgc:56136) [Danio rerio] 6.0E-28 20 6 10 36808, 002867 B5405 49.24 385.05 0.47 JGI_292814 ATP synthase alpha-subunit [Ciona intestinalis] (mitochondrialF1 complex) 0.0E+00 33 8 4 003968 A6112 35.03 382.40 0.56 Not determined9 B4204 38.12 372.64 0.27 JGI_216024 isocitrate dehydrogenase 3, beta subunit [Mus musculus] 3.0E-122 20 6 0 0424810 A6012 30.78 324.06 0.70 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645611 A7302 50.50 303.82 0.44 JGI_298739 mitochondrial citrate synthase precursor [Xiphias gladius] 0.0E+00 38 3 2 02113, 0080912 A5314 48.77 301.30 0.44 KYOTOGRAIL2005.45.49.1 phosphoglycerate kinase [Halocynthia roretzi] 1.0E-171 7 2 1 0567613 A8001 29.89 266.87 0.33 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645614 A8004 28.55 256.28 0.57 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645615 B4203 40.12 248.23 0.98 KYOTOGRAIL2005.297.4.1 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase)(Acetyl-CoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA 5.0E-135 10 9 0 00534, 01655316 B7913 138.65 237.13 0.35 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645617 B9201 37.08 236.78 1.06 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645618 B4107 34.99 233.87 1.03 PROCITS_00881_0_1_0 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 2.0E-154 49 54 19 00881, 37267, 3613919 A4107 37.55 224.03 0.45 KYOTOGRAIL2005.26.37.1 malate dehydrogenase, mitochondrial [Rattus norvegicus] 3.0E-127 18 9 1 0466820 A1005 29.57 205.82 0.29 JGI_224382 ATP synthase, H+ transporting, mitochondrial F0 complex,subunit b, isoform 1 [Danio rerio] 3.0E-34 34 22 1 0003421 B7101 31.46 190.66 0.66 KYOTOGRAIL2005.545.3.1 Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2) 7.0E-89 38 61 4 00121, 3661322 B9002 24.69 187.74 0.75 KYOTOGRAIL2005.195.15.1 No similarity 4 14 0 0308123 A2104 38.41 181.17 0.41 PROCITS_00085_1_1_0 Rplp0 protein [Danio rerio] ribosomal P10 protein 1.0E-111 145 149 217 1358424 A7206 41.98 167.12 0.29 JGI_294238 isocitrate dehydrogenase 3 (NAD+) alpha [Mus musculus] 4.0E-158 25 9 0 0442225 B7103 31.51 165.41 1.55 KYOTOGRAIL2005.545.3.1 Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2) 7.0E-89 38 61 4 00121, 3661326 B5002 23.87 163.82 3.94 KYOTOGRAIL2005.416.5.2 ATP synthase, H+ transporting, mitochondrial F0 complex,subunit d [Danio rerio] 4.0E-24 15 21 2 05392, 16890, 1676727 A201 41.87 159.40 0.42 PROCITS_00526_1_1_0 67kD laminin receptor precursor [Pinctada fucata] 2.0E-107 20 5 6 00526, 0957628 A405 54.28 159.01 0.38 KYOTOGRAIL2005.174.27.1 tubulin, beta 4 [Xenopus tropicalis] 0.0E+00 82 43 85 00086,13299,1436029 A407 54.82 151.75 0.39 JGI_206517 alpha-tubulin 1 [Drosophila melanogaster] 0.0E+00 33 29 2 0234030 A6811 107.93 142.26 0.20 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645616 cell-stage embryosTable 2. Continued1 B8401 44.94 572.31 0.08 KYOTOGRAIL2005.55.42.3 translation elongation factor 1A 8.0E-170 103 189 187 05079, 14095, 13525,00080, 103212 A2209 45.10 488.82 0.23 PROCITS_00805_1_2_0 actin [Ciona intestinalis] 0.0E+00 0 0 0 000463 B7001 24.70 480.47 1.53 KYOTOGRAIL2005.216.2.1 H+ transporting ATP synthase O subunit isoform 1 [Bombyx 1.0E-37 19 16 3 02586, 017364 B7002 26.93 351.37 0.40 KYOTOGRAIL2005.119.5.1 novel protein (zgc:56136) [Danio rerio] 6.0E-28 20 6 10 36808, 002865 B9202 37.17 340.83 0.30 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 164566 B4204 38.12 310.93 0.10 JGI_216024 isocitrate dehydrogenase 3, beta subunit [Mus musculus] 3.0E-122 20 6 0 042487 A4107 37.55 302.17 0.39 KYOTOGRAIL2005.26.37.1 malate dehydrogenase, mitochondrial [Rattus norvegicus] 3.0E-127 18 9 1 046688 A7302 50.50 252.45 0.12 JGI_298739 mitochondrial citrate synthase precursor [Xiphias gladius] 0.0E+00 38 3 2 02113, 008099 B5002 23.87 238.36 1.34 KYOTOGRAIL2005.416.5.2 ATP synthase, H+ transporting, mitochondrial F0 complex,subunit d [Danio rerio] 4.0E-24 15 21 2 05392, 16890, 1676710 B9201 37.08 210.16 0.02 KYOTOGRAIL2005.100.11.1 No similarity (Vitellogenin) NA NA NA NA11 A5007 30.17 208.82 0.28 Not determined12 A2104 38.41 205.66 0.18 PROCITS_00085_1_1_0 Rplp0 protein [Danio rerio] ribosomal P10 protein 1.0E-111 145 149 217 1358413 A1308 45.83 205.41 0.49 PROCITS_10733_1_1_0 Actin, muscle 0.0E+00 0 1 36868, 1073314 A1005 29.57 192.67 0.12 JGI_233126 No similarity 34 22 1 0003415 A1405 55.40 191.95 0.34 KYOTOGRAIL2005.274.14.1 ATP synthase, H+ transporting mitochondrial F1 complex, betasubunit [Mus musculus] 0.0E+00 63 20 1 0303116 B5405 49.24 188.28 0.11 JGI_292814 ATP synthase alpha-subunit [Ciona intestinalis] (mitochondrialF1 complex) 0.0E+00 33 8 4 0039617 A8008 28.98 169.20 0.26 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18 0 0 0 00997, 1645618 A7206 41.98 166.42 0.13 JGI_294238 isocitrate dehydrogenase 3 (NAD+) alpha [Mus musculus] 4.0E-158 25 9 0 0442219 B7103 31.51 159.05 0.68 KYOTOGRAIL2005.545.3.1 Voltage-dependent anion-selective channel protein 2 (VDAC-2) (hVDAC2) (Outer mitochondrial membrane protein porin 2) 7.0E-89 38 61 4 00121, 3661320 B9602 70.65 149.49 0.39 KYOTOGRAIL2005.116.8.1 mitochondrial trifunctional protein, alpha subunit [Xenopus 0.0E+00 15 5 0 01869,0270121 B9002 24.69 148.75 0.03 KYOTOGRAIL2005.195.15.1 No similarity 4 14 0 0308122 A8208 45.01 143.36 0.59 JGI_208156 aldolase C, fructose-bisphosphate [Pan troglodytes] 4.0E-139 5 4 30 0009423 B9004 24.71 137.92 2.19 Not determined24 B4107 34.99 130.93 0.22 PROCITS_00881_0_1_0 Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) 2.0E-154 49 54 19 00881, 37267, 3613925 A5001 26.72 124.92 0.62 JGI_291517 peroxiredoxin-like [Ciona intestinalis] 6.0E-112 6 4 18 0013826 A7104 32.20 124.20 0.44 KYOTOGRAIL2005.182.19.1 Electron-transfer-flavoprotein, beta polypeptide [Xenopus 1.0E-90 12 2 1 14809, 0568327 A5306 49.68 119.19 0.05 KYOTOGRAIL2005.45_49_1 phosphoglycerate kinase [Halocynthia roretzi] 1.0E-171 7 2 1 0567628 A3307 51.66 118.66 0.29 KYOTOGRAIL2005.289.16.1 Alpha-enolase (2-phospho-D-glycerate hydro-lyase) (Non-neural enolase) (NNE) (Enolase 1) 3.0E-176 3 3 5 03247, 04432, 0166229 A5010 29.35 118.00 0.29 KYOTOGRAIL2005.103.6.1 glutathione peroxidase [Suberites domuncula] 1.0E-73 9 5 1 0286930 A2108 32.39 114.84 0.04 JGI_233410 prohibitin [Homo sapiens] 1.0E-74 12 10 0 01295Tadpole larvaeCLUSTER Clones Description Sequence ID SSN QTYCLSTR00046 162/29444 cytoplasmic actin 1 PROCITS_00805_1_2_0 A2209 349.66CLSTR00424 146/29444 no similarity not detected.CLSTR00085 144/29444 acidic ribosomal phosphoprotein PROCITS_00085_1_1_0 A2104 143.81CLSTR00333 141/29444 cytochrome P450 monooxygenase not detected.CLSTR00032 136/29444 no similarity not detected.CLSTR00020 135/29444 ATP/ADP translocase not detected.CLSTR00585 112/29444 C9 not detected.CLSTR00081 112/29444 ribosomal protein S1a not detected.CLSTR00080 103/29444 translational elongation factor-1 alpha KYOTOGRAIL2005.55.42.3 B8401 431.68CLSTR00032 365 / 26796 no similarity not detected.CLSTR00080 189 / 26796 translational elongation factor-1 alpha KYOTOGRAIL2005.55.42.3 B8401 1051.94CLSTR00020 173 / 26796 ATP/ADP translocase not detected.CLSTR00046 149 / 26796 actin 1 PROCITS_00805_1_2_0 A2209 398.13CLSTR00085 149 / 26796 acidic ribosomal phosphoprotein PROCITS_00085_1_1_0 A2104 181.16CLSTR00081 147 / 26796 ribosomal protein S1a not detected.CLSTR00424 143 / 26796 no similarity not detected.CLSTR01131 125 / 26796 Splicing factor, arginine/serine-rich 7 not detected.CLSTR01468 125 / 26796 Meprin A beta-subunit precursor not detected.CLSTR00734 97 / 26796 G2/mitotic-specific cyclin B1 not detected.CLSTR00065 1488/25532 no similarity not detected.CLSTR00046 937/25532 cytoplasmic actin PROCITS_00805_1_2_0 A2209 488.12CLSTR00087 395/25532 no similarity not detected.CLSTR00419 363/25532 no similarity not detected.CLSTR00025 290/25532 muscle actin not detected.CLSTR00085 217/25532 acidic ribosomal protein P0 PROCITS_00085_1_1_0 A2104 205.66CLSTR00080 184/25532 translational elongation factor-1 alpha KYOTOGRAIL2005.55.42.3 B8401 572.31CLSTR00081 169/25532 40S ribosomal protein not detected.CLSTR00359 139/25532 troponin T not detected.Table 3.  Genes with high occurrence and their corresponding proteins identified in each embryonic stage.TadpolelarvaeCleavingembryosUnfertilized eggsTable 4. Protein spots unique in unfertilized eggs, 16 cell-stage embryos and tadpole larvae.SSN Mr(observed)QTY C.V. Sequence ID Description E-valueUnfertilized eggsB6205 39.42 26.90 0.13 JGI_238339 Glutamic-oxaloacetic transaminase 2b, mitochondrial (aspartateaminotransferase 2) [Danio rerio] 6.0E-170B4302 42.69 17.56 0.38 not determinedB5503 63.51 9.24 0.66 not determinedB6605 68.74 7.87 0.39 KYOTOGRAIL2005.221.21.1 ATPase family, AAA domain containing 3A [Xenopus tropicalis] 0.0E+00A4207 43.58 7.00 0.63 KYOTOGRAIL2005.253.11.1 Isovaleryl Coenzyme A dehydrogenase [Danio rerio] 3.0E-17516 cell-stage embryosA6112 35.03 382.40 0.56 not determinedB8201 34.56 76.06 1.12 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18B8403 44.07 63.32 1.89 not determinedB3208 37.36 39.89 0.71 KYOTOGRAIL2005.151.18.3 myosin, light chain kinase [Gallus gallus] 0.0E+00B7401 43.90 38.47 2.70 not determinedB3306 42.96 27.53 0.98 not determinedB5304 41.65 18.42 0.80 not determinedA2105 38.99 13.70 0.34 not determinedB4408 44.44 11.22 1.99 not determinedA1101 36.15 2.94 0.99 not determinedTadpole larvaeA5007 30.17 208.82 0.28 not determinedA5306 49.68 119.19 0.05 KYOTOGRAIL2005.45.49.1 phosphoglycerate kinase [Halocynthia roretzi] 1.0E-171A3504 62.70 105.63 0.14 not determinedA3104 38.97 97.97 0.52 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 6.0E-18B5201 40.17 87.00 0.70 KYOTOGRAIL2005.297.4.1 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (AcetylCoA acyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase)- 5.0E-135B4105 34.66 74.28 0.42 not determinedA2502 63.52 66.78 0.16 KYOTOGRAIL2005.100.11.1 No similarity (Vitellogenin)A5107 32.78 51.79 0.54 N. D.A5713 95.06 50.10 0.13 KYOTOGRAIL2005.100.11.1 No similarity (Vitellogenin)A4105 32.68 49.34 0.62 not determinedSSNs are spot unique identification numbers that are automatically assinged to each spot by 2-DE anaylis software (see Materials and Methods). SSNs beggining with "A" indicate spots in pH4-72-DE gel, and those beggining with "B" indicate spots in pH6-11.Table 5. Up-regulated proteins in the embryogenesis in C. intestinalis.SSN  Sequence ID Description Change in QTY(-fold change)Egg to 16cell1 B4203 KYOTOGRAIL2005.297.4.1 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoAacyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) 4.982 B5401 KYOTOGRAIL2005.353.8.1 leucyl aminopeptidase, putative [Aedes aegypti] 4.843 B4406 Not determined 4.814 A5602 JGI_293460 ezrin/radixin/moesin (ERM)-like protein [Ciona intestinalis] 3.475 A2 Not determined 2.976 B9302 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 2.957 B4403 KYOTOGRAIL2005.198.6.1 fascin 1(55 kDa actin-bundling protein) (p55) [Homo sapiens] 2.958 B3201 KYOTOGRAIL2005.297.4.1 3-ketoacyl-CoA thiolase, mitochondrial (Beta-ketothiolase) (Acetyl-CoAacyltransferase) (Mitochondrial 3-oxoacyl-CoA thiolase) 2.939 A3603 Not determined 2.7810 A8506 Not determined 2.7216cell to Tadpole larva1 A1308 PROCITS_10733_1_1_0 Actin, muscle 30.772 A1210 PROCITS_00249_0_1_0 ATP-specific succinyl-CoA synthetase beta subunit [Mus musculus] 29.133 A8307 Not determined 25.284 A1609 JGI_210507 Plastin-3 (T-plastin) 20.725 A2602 Not determined 20.426 A7306 Not determined 20.017 A4209 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 17.368 A4304 KYOTOGRAIL2005.160.3.1 PREDICTED: similar to protein disulfide isomerase-associated 3 [Ciona intestinalis] 16.709 A5310 Not determined 14.3510 B7304 Not determined 13.64SSNs are spot unique identification numbers that are automatically assinged to each spot by 2-DE anaylis software (see Materials and Methods). SSNs beggining with "A" indicate spots in pH4-7 2-DEgel, and those beggining with "B" indicate spots in pH6-11.Table 6. Down-regulated proteins in the embryogenesis in C. intestinalis.SSN  Sequence ID Description Change in QTY(-fold change)Egg to 16cell1 A1210 PROCITS_00249_0_1_0 ATP-specific succinyl-CoA synthetase beta subunit [Mus musculus] 0.022 A4304 KYOTOGRAIL2005.160.3.1 PREDICTED: similar to protein disulfide isomerase-associated 3 [Ciona intestinalis] 0.083 A2408 KYOTOGRAIL2005.45.31.1 Chaperonin containing TCP1, subunit 2 (beta) [Xenopus tropicalis] 0.104 A2307 Not determined 0.105 A2509 KYOTOGRAIL2005.117.37.1 programmed cell death 8 [Gallus gallus] 0.156 A7109 Not determined 0.167 A4507 PROCITS_00049_1_1_1 T-complex protein 1 subunit gamma (TCP-1-gamma) (CCT-gamma) 0.188 A5313 KYOTOGRAIL2005.53.53.1 fumarate hydratase [Gallus gallus] 0.289 A7604 JGI_375252 Glycyl-tRNA synthetase [Mus musculus] 0.3110 B4501 KYOTOGRAIL2005.100.2.1 No similarity (Vitellogenin) 0.3216cell to Tadpole larva1 A7211 KYOTOGRAIL2005.100.2.1 No similarity (Vitellogenin) 0.002 A7803 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 0.043 A5101 KYOTOGRAIL2005.269.21.1 Enoyl Coenzyme A hydratase, short chain, 1, mitochondrial [Xenopus tropicalis] 0.044 A6811 JGI_286147 apolipoprotein B [Rattus norvegicus] (Vitellogenin) 0.105 B4504 KYOTOGRAIL2005.100.2.1 No similarity (Vitellogenin) 0.116 A7508 Not determined 0.137 A1605 Not determined 0.158 A2202 Not determined 0.189 B4406 Not determined 0.21KYOTOGRAIL2005.53.53.1 fumarate hydratase [Gallus gallus] 0.22JGI_252770 elongation factor-1 gamma [Diguetia canites] 0.2810 A5313*

Proteomic profiles of embryonic development in the ascidian Ciona intestinalis

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