Abstract Background The repeated use of navigation assistance systems leads to decreased spatial orienting abilities. Previous studies demonstrated that augmentation of landmarks using auditory navigation instructions can improve incidental spatial learning when driving on a single route through an unfamiliar environment. Objective Based on these results, a series of experiments was conducted to further investigate both the impairment of spatial knowledge acquisition by standard navigation instructions and the positive impact of landmark augmentation in auditory navigation instructions on incidental spatial learning. Method The first Experiment replicated the previous setup in a driving simulator without additional visual route indicators. In a second experiment, spatial knowledge was tested after watching a video depicting assisted navigation along a real-world urban route. Finally, a third Experiment investigated incidental spatial knowledge acquisition when participants actively navigated through an unrestricted real-world,urban environment. Results All three experiments demonstrated better cued-recall performance for participants navigating with landmark-based auditory navigation instructions as compared to standard instructions. Notably, standard instructions were associated with reduced learning of landmarks at navigation relevant intersections as compared to landmarks alongside straight segments and the recognition of novel landmarks. Conclusion The results revealed a suppression of spatial learning by established navigation instructions, which were overcome by landmark-based navigation instructions. This emphasizes the positive impact of auditory landmark augmentation on incidental spatial learning and its generalizability to real-life settings. Application This research is paving the way for navigation assistants that, instead of impairing orienting abilities, incidentally foster spatial learning during every-day navigation. Précis This series of three experiments replicates the suppression of spatial learning by standard navigation instructions and the positive impact of landmark augmentation in auditory navigation instructions on incidental spatial learning during assisted navigation. Three experiments with growing degree of realism revealed the applicability and generalizability to real-life settings
