Eight endurance~trained cyclists rode as far as possible in 1 h on a stationary cyclesimulator in a moderate environment (20°C, 60% relative humidity, 3 m/s wind speed) while randomly receiving either no fluid (NF) or attempting to replace their ~1.7 l sweat loss measured in a previous 1 h familiarisation performance ride at ~85% of peak oxygen uptake (VO₂ peak) with artificially sweetened, coloured water (F). During F the cyclists drank 1.49 ± 0.14 1 (values are mean± SEM), of which 0.27 ± 0.08 1 remained in the stomach at the end of exercise and 0.20 ± 0.05 1 was urinated after the trial. Thus, only 1.02 ± 0.12 l of the ingested fluid was available to replace sweat losses during the 1 h performance ride. That fluid decreased the average heart rate from 166 ± 3 to 157 ± 5 beats/min (P 1.0 l/h sweat losses during high-intensity, short duration exercise in a moderate environment does not induce beneficial physiological effects, and may impair exercise performance