The Catalytic Machinery of Chondroitinase ABC I Utilizes a Calcium Coordination

Abstract

The chondroitinases are bacterial lyases that specifically cleave chondroitin sulfate and/or dermatan sulfate glycosaminoglycans. One of these enzymes, chondroitinase ABC I from Proteus Vulgaris, has the broadest substrate specificity and has been widely used to depolymerize these glycosaminoglycans