Analyzing mRyzingCF:3 complexes using a yeast

Abstract

RR)C)C)C) interactions are essential for the proper execution and regulation of every step in the life of a eukaryoticmRka Here we describe a three-hybrid system in whichRchC[1FI[C interactions can be analyzed using simple phenotypic or enzymatic assays in Saccharomyces cerevisiae. The system can be used to detect or confirm anR1:F]FCE1: interaction, to analyzeRal protein interactions genetically, and to discover new protein or RC partners when only one is known. Multicomponent complexes containing more than one protein can be detected, identified, and analyzed. We describe the method and how to use it, and discuss applications that bear particularly on eukaryoticmRkar # 2002 Elsevier Science (USA). All rights reserved