The species of spiders (Arachnida Araneae) found in the “Isola delle Femmine Nature Re-
serve” (Sicily, Italy) are reported here. The checklist provided is the result of some excursions
carried out between 2021 and 2023 and aimed at studying the invertebrate fauna that lives
on this small island. In total, up to date, 42 species, belonging to 38 genera and 19 families,
are listed, among these Diplostyla concolor (Wider, 1834) is reported for the first time for
Sicily