DOI:10.2298/ABS0701001S EFFECT OF THE KANAMYCIN RESISTANCE MARKER ON STABILITY OF 2m-BASED EXPRESSION PLASMIDS

Abstract

Abstract – In this paper we describe the effect of the kanamycin resistance gene (Km r) on 2μm-based plasmid maintenance in Saccharomyces cerevisiae. The influence of this marker gene on the loss of the stable model-vectors proved to be constant, as well as independent of carbon source and culture growth rates. In strains for GAL UAS – driven heterologous protein production introduction of Km r resulted in curing of the yeast episomal plasmid (YEp) from the population in a small number of generations. Application of selective pressure on the strain producing recombinant penicillin G amidase (rPGA) did not provide the expected increase of protein yield. The influence of genetic elements for heterologous protein production on vector stability was examined, and the most destabilizing factors prove to be the presence and expression of the foreign gene