Conserved Mechanism of Negative Gene Regulation by Extracellular Calcium Parathyroid Hormone Gene versus Atrial

Abstract

We found that a negative calcium-responsive element (nCaRE) originally reported in the human parathyroid hormone gene is conserved among several genes. The results of the present study show that expression of one of the genes, the rat atrial natriuretic polypeptide gene, was negatively regulated in the heart by extracellular calcium by using an in vivo infusion system. Moreover, transfection of the cultured cells revealed that this DNA element conferred negative regulation by extracellular calcium on the reporter gene. It is suggested that there is a gene family whose expression is negatively regulated by extracellular calcium through this conserved DNA motif, nCaRE. (J. Clin. Invest. 1992.89:1268-1273.) Key words: negative calcium-responsive element * in vivo infusion system * transfection * gene family * nuclear protei