EFFECT OF MEDIUM pH AND CULTIVATION PERIOD ON MYCELIAL BIOMASS, POLYSACCHARIDE, AND LIGNINOLYTIC ENZYME PRODUCTION BY GANODERMA LUCIDUM FROM MONTENEGRO

Abstract

Abstract- The effect of initial medium pH on biomass, extracellular and intracellular polysaccharide, and ligninolytic enzyme production by Ganoderma lucidum was investigated at different pH values after 7 and 14 days of cultivation. Maximal production of biomass was recorded at pH 4.5 and 5.0; maximal production of extracellular polysaccharides at pH 7.0 and 3.0; and maximal production of intracellular polysaccharides at pH 7.0 and 5.5. Ligninolytic enzymes were not produced at any pH of the medium. Maximal biomass production was obtained on the 11 th day of cultivation; maximal extracellular polysaccharide production on the 7 th day; and maximal intracellular polysaccharide production on the 6 th and 10 th day of cultivation