In the present study the macroinvertebrate composition in the Misoča River, its diversity and abundance were analyzed. The study was conducted in autumn and spring, in order to determine the influence of seasonal dynamics on the structure of macroinvertebrate communities and water quality. A biotic element analysis revealed a high abundance and diversity of macroinvertebrate taxa. Significant differences in the structure of communities with respect to seasons and sampling sites were observed, as well as in species dominance, which was confirmed by the Bray-Curtis similarity analysis. The results of the EPT% index, EPT/Chironomidae index, Shannon-Weaver and Simpson\u27s biodiversity indices showed the good ecological status of water at studied sites. The Pantle-Buck saprobic index indicated an oligo to β-mesosaprobic category, or slightly contaminated water. The presence of the Natura 2000 species Austropotamobius torrentium, and of six Trichoptera species new to the fauna of Bosnia and Herzegovina and, among others, the species Hydropsyche botosanaenui was recorded in samples. This study represents the first literature data on macroinvertebrate diversity for the protected area of the Misoča River
