Ramji Gupta et.al Indian Journal of Research in Pharmacy and Biotechnology ISSN: 2321-5674(Print) ISSN: 2320 – 3471(Online) Anti-osteoporotic effect of Urtica dioica on ovariectomised rat

Abstract

This study was undertaken to check the anti-osteoporotic activity of Urtica dioica on ovariectomised (OVX) rats. Bilateral ovariectomy was performed in rats under aseptic condition and ether anaesthesia. Healthy albino rats were randomly divided in to 4 groups of 6 animals each. First group Assigned by sham operated and served as control, all remaining are Ovariectomy. Group (II) is ovearctomised control, group (III) Urtica dioica extract (20mg/kg), group (IV) treated with Raloxifene (5.4mg/kg as standard drug) respectively for 8 weeks. At the last of study blood wear collected to estimate serum calcium (Ca), phosphorus (Ph), bone specific alkaline phosphate (b-Alp) and osteocalcin (OC) level. Rats were euthanized femur, liver and uteri wear taken for bone analysis and weighed respectively. Result indicate that ovariectomy(OVX) gp show increased serum calcium (Ca), phosphorus (Ph), bone specific alkaline phosphate (b-Alp) and osteocalcin (OC) level also decreased Bone Mineral Density (BMD), Ca + in bone ash. Femur and uterine wear weighted. When OVX gp treated with Urtica dioica (200mg/kg) it normalise all elevated serum calcium (Ca), phosphorus (Ph), bone specific alkaline phosphate (b-Alp) and osteocalcin (OC) level and increased femur, uterine weight, it also increased BMD and Bone Volume. This study assess on the basis of biochemical, biomechanical parameter which show that administration of Urtica dioica is beneficial for women who are suffering for postmenopausal osteoporosis due to its good antiosteoporotic activity. KEY WORDS: Ovariectomised rats, osteocalcin, alkaline phosphate, BMD, Bone fragility 1