Building a Simplistic Automatic Extruder: Instrument Development Opportunities for the Laboratory

Abstract

A well-rounded introduction to work in a STEM laboratory is vital to scientific education. Besides the ability to use available instrumentation for sample characterization, students should also be imparted knowledge in the steps of instrument development and construction. These concepts can be taught using the example of lipid vesicle preparation via extrusion. Vesicle extrusion is a common technique that involves syringes pushing solutions through membrane filters and is used in fundamental studies on vesicles. Such research is important to better understand of biological phenomena and drug development. Well prepared samples are key to successful research. While the manual approach is very useful to acquire experience, automatic extrusion is more convenient, and automation often results in better reproducibility. These advantages can be combined in a simplistic automatic extruder, that does not require advanced technical skills to be assembled. It can therefore be used by various groups, ranging undergraduate to graduate students using equipment typically available. Using this approach, students can acquire different skillsets including coding, testing, and advanced use of building materials based on their properties. Finally, the quality of the automatic extruder is verified.Comment: 21 pages supplementary information included, 11 Figures and a cover picture, LabVIEW workflow file can be found on the group's gitHub profil