Hohmann Judit

Maaz Mohamed

Vasas Andrea

English

University of Szeged

5th Symposium of Young Researchers on PharmacognosySzeged, 23 July 2024BOOK OF ABSTRACTS5th Symposium of Young Researchers on Pharmacognosy BOOK OF ABSTRACTS (ed. Tivadar Kiss, Judit Hohmann) Institute of Pharmacognosy, University of Szeged, Szeged, Hungary  23 July 2024      doi: 10.14232/syrpharmacognosy.2024.af 5th Symposium of Young Researches on Pharmacognosy14A8  Isolation and structure elucidation of compounds from Euphorbia systyloides Mohamed Maaz1, Judit Hohmann1,2, Andrea Vasas1,2 1   Email: mohamedmaaz50@gmail.com Euphorbiaceae, a diverse plant family, includes large desert succulents, trees, and small herbaceous types. Numerous species produce toxic milky juice and exhibit significant medicinal properties [1]. Euphorbia species are notable for their anti-inflammatory and anticancer active constituents, particularly diterpenes and triterpenes [2]. This work aims at the isolation, structure elucidation, and pharmacological investigation of specialized plant metabolites, especially diterpenes and triterpenes from Euphorbia species. Euphorbia systyloides is a toxic, tropical plant that has never been investigated from phytochemical or pharmacological points of view. The methanol extract obtained from the aerial parts of the plant was evaporated in vacuo, dissolved in 50% methanol, and then subjected to solvent solvent partition with n-hexane, chloroform, and ethyl acetate, respectively. The antiproliferative activity of the three fractions was tested in vitro against COLO 205, COLO 320, and HeLa cell lines. The chloroform phase was then further purified by different chromatographic methods, vacuum column chromatography (VCC), flash chromatography (FC), and finally reversed-phase high-performance liquid chromatography (RP-HPLC). 1D and 2D NMR spectra were recorded in methanol-d4 on a Bruker Avance DRX 500 spectrometer at 500 MHz (1H) and 125 MHz JMOD (13C). So far, three tirucallane triterpenes have been isolated, among them two novel compounds. Furthermore, one gallic acid and a megastigmane derivative were also isolated from the plant. Acknowledgements: This work was supported by the National Research, Development and Innovation Office, Hungary (NKFIH; K-134704). References [1] Ramalho SD et al. Planta Med. 2018, 84(9-10):558 567. [2] - Cancers (Basel). 2023, 16(1):114.  

Isolation and structure elucidation of compounds from Euphorbia systyloides : [abstract]

http://acta.bibl.u-szeged.hu/85200/1/syrpharmacognosy_2024_014.pdf

Isolation and structure elucidation of compounds from Euphorbia systyloides : [abstract]

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