Depletion of deepwater dissolved oxygen (DO) in lakes has become increasingly prevalent and severe because of many external stressors, potentially threatening human-derived ecosystem services ranging from drinking water quality to fisheries. Using year-round, high-frequency DO data from 12 dimictic lakes, we compared 3 measures of deepwater DO depletion during winter and summer: DO depletion rate, DO minimum, and hypoxia duration. Hypoxia (DO 65%) regardless of the previous summer’s DO depletion severity. This lack of ecological memory in deepwater DO depletion across seasons suggests that deepwater DO largely resets during spring and fall mixing periods in most years in these dimictic lakes. Understanding the patterns and drivers in deepwater DO depletion in both winter and summer is a key step forward for predicting future chemical and biological consequences of seasonal DO depletion and managing lake ecosystem health, as well as the effects that climate change may have on these patterns.acceptedVersio