Costa, SS

Couto, I

Feßler, Andrea T

Hanke, D.

Krüger-Haker, H.

Morais, C

Pomba, Constança

Santos, A.

Schwarz, S,

English

Repositório da Universidade Nova de Lisboa

CONGRESS OF MICROBIOLOGY AND BIOTECHNOLOGY 2023BOOK OF7TH - 9THDECEMBERABSTRACTSUNIVERSIDADE DA BEIRA INTERIORCovilhãCongress of Microbiology and Biotechnology 2023  431             MicroBiotec 2023 - Covilhã, Portugal  P4.49 - PLASMID PROFILING OF STAPHYLOCOCCUS PSEUDINTERMEDIUS ASSOCIATED WITH SKIN AND SOFT TISSUE INFECTIONS IN COMPANION ANIMALS   Catarina Morais 1(*), Sofia Santos Costa 1, Dennis Hanke 2,3, Henrike Krüger-Haker 2,3, Ana Santos 1, Constança Pomba 4, Andrea T. Feβler 2,3, Stefan Schwarz 2,3, Isabel Couto 1 1 Global Health and Tropical Medicine, GHTM, Associate Laboratory in Translation and Innovation Towards Global Health, LA-REAL, Instituto de Higiene e Medicina Tropical, IHMT, Universidade NOVA de Lisboa, UNL, Lisbon, Portugal 2 Institute of Microbiology and Epizootics, Centre for Infection Medicine, School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany 3 Veterinary Centre for Resistance Research (TZR), School of Veterinary Medicine, Freie Universität Berlin, Berlin, Germany 4 Laboratory of Antimicrobial and Biocide Resistance, CIISA, Faculty of Veterinary Medicine, University of Lisbon, Lisbon, Portugal  (*) e-mail: a21000758@ihmt.unl.pt   Keywords: Staphylococcus pseudintermedius; plasmid; antimicrobial resistance; whole-genome sequencing.    ABSTRACT The increasing antimicrobial resistance (AMR) of staphylococci causing skin and soft-tissue infections (SSTIs) in companion animals is a public health concern. In this study, we analyzed the plasmid content and the associated antimicrobial resistance profiles in relevant clonal lineages of S. pseudintermedius causing SSTIs in companion animals. The study focused on 41 S. pseudintermedius, representing predominant and emerging clonal lineages associated with SSTIs in dogs and cats collected in Lisbon (Portugal), previously characterized regarding antimicrobial resistance and clonality1. Plasmid DNA was extracted, digested with XbaI restriction enzyme and restriction patterns analyzed in agarose gel electrophoresis. Plasmids were classified according to their predicted molecular weight as low (≤ 3 kb), medium (3 - 10kb), or high-molecular weight (≥ 23 kb) plasmids. Each unique restriction pattern was assigned to a plasmid profile. A subset of 15 strains was further analyzed by hybrid WGS (MinION, Illumina). Twenty-five out of the 41 (61%) representative S. pseudintermedius isolates carried one or more plasmids, most of medium or high molecular weight, that corresponded to eleven plasmid profiles. Strains from relevant MLST sequence-types (ST) carried plasmids, namely ST71 (17/24), ST241 (3/3), ST157 (1/4), ST118, ST258, ST265 and ST551 (each 1/1). No plasmids were detected among ST45 strains (3/3). Ongoing WGS data analyses will allow deeper insight into S. pseudintermedius genomes and to establish relations between antimicrobial resistance phenotypes and the mobilome. The rapid transfer of mobile genetic elements such as the plasmids studied here could boost the increasing AMR in S. pseudintermedius and other related species, such as S. aureus.  References: 1. Morais C, et al. (2023). Front.Microbiol.14:1167834. doi:10.3389/fmicb.2023.1167834  Congress of Microbiology and Biotechnology 2023  432             MicroBiotec 2023 - Covilhã, Portugal   Acknowledgements:  Project BIOSAFE funded by FEDER and Fundação para a Ciência e a Tecnologia (FCT, Portugal), Grant LISBOA-01-0145-FEDER-030713, PTDC/CAL-EST/30713/2017. Further support by FCT to GHTM (UID/04413/2020), LA-REAL (LA/P/0117/2020), grant UI/BD/151061/2021 and Bilateral PT Germany (FCT/DAAD) mobility project 2022.15300.CBM.     

Plasmid profiling of Staphylococcus pseudintermedius associated with skin and soft tissue infections in companion animals

https://run.unl.pt/bitstream/10362/166330/1/MoraisC_MicroBiotec23_Poster.pdf

Plasmid profiling of Staphylococcus pseudintermedius associated with skin and soft tissue infections in companion animals

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