Isoflavones, a significant class of plant polyphenols, are recognized for their health benefits, including cholesterol reduction, cancer prevention, and mitigation of osteoporosis and menopause symptoms. This study focuses on optimizing the supercritical fluid extraction (SFE) of isoflavones from kudzu (Pueraria montana) and red clover (Trifolium pratense) flowers, which are rich in these compounds. The extraction process was optimized by varying pressure, time, and co-solvent types. Comparisons were made with ultrasound-assisted extraction (UAE) using natural deep eutectic solvents (NADES) and conventional extraction methods to evaluate yield, antioxidant activities, total phenolic content, and total flavonoid content in the extracts.The research was supported by the grant of the Russian Science Foundation № 24-25-20147 (https://rscf.ru/project/24-25-20147) and the Government of the Sverdlovsk region
