Health microbiological pig meat: evaluation of the antimicrobial resistance of Escherichia coli

Abstract

El hallazgo de cepas resistentes de E. coli en el ganado porcino ha sido estudiado en Argentina; pero falta investigar si estas cepas pueden llegar al consumidor. El objetivo del presente trabajo fue determinar la prevalencia de cepas de E. coli antibiótico resistentes en carnes porcinas frescas de canales de venta minorista de La Plata, Buenos Aires. Se estudiaron carnes de 47 puestos. Los estudios microbiológicos se realizaron de acuerdo a la información proporcionada por el National Comittee for Clinical Laboratory Standards para microorganismos aislados de animales, para inferir la resistencia y sensibilidad. Las carnes se procesaron en Stomacker según lo recomendado por CAA. Se utilizó caldo peptonado y EMB, incubando 24 horas a 35 ºC. Las colonias sospechosas de E. coli se tipificaron con metodología convencional. La prueba de sensibilidad antimicrobiana se efectuó según el método de dilución en agar (Kirby Bauer) bajo las normas NCCLS. De las 10 cepas de E. coli aisladas (21 % del total de carnes), 7 mostraron susceptibilidad a nitrofurantoína (NIT), aminotriptilina + sublactama (AMS), norfloxacina (NOR), gentamicina (GEN), trimetoprima-sulfametoxazol (TMS) y cefalotina (CEF). Por otra parte, 3 cepas (30 % de los aislamientos de E. coli detectados) resultaron susceptibles a NIT, AMS, NOR, GEN, TMS, pero resistentes a CEF.The finding of resistant strains of E. coli in pigs has been studied in Argentina, but need to investigate whether these strains can reach the consumer. The aim of this study was to determine the prevalence of resistant E. coli antibioticresistance in fresh pig meat retail channels in La Plata, Buenos Aires. Argentine. Meat were studied 47 posts. Microbiological studies were carried out according to information provided by the National Committee for Clinical Laboratory Standards for animal isolates, to infer the strength and sensitivity. The meat will be processed in stomack as recommended by CAA. Peptone broth was used and EMB, incubated 24 hours at 35 ºC. Suspected colonies of E. coli were typed by conventional methodology. Antimicrobial susceptibility testing was performed according to the agar dilution method (Kirby Bauer) under NCCLS standards. Strains of E. coli were resuspended in peptone water. Of the 10 strains of E. coli isolates (21% of total meat), seven were susceptible to nitrofurantoin (NIT), aminotriptilina + sublactama (AMS), norfloxacin (NOR), gentamicin (GEN), trimethoprim-sulfamethoxazole (TMS) and cephalothin (CEF). Moreover, 3 strains (30% of the isolates of E. coli detected) were susceptible to NIT, AMS, NOR, GEN, TMS, but resistant to CEF.Facultad de Ciencias Agrarias y Forestale

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