Dataset related to the article "Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis"

Abstract

<p>This record contains raw data related to the article “Prenylcysteine Oxidase 1 Is a Key Regulator of Adipogenesis"</p> <p>Abstract: The process of adipogenesis involves the differentiation of preadipocytes into mature<br> adipocytes. Excessive adipogenesis promotes obesity, a condition that increasingly threatens global<br> health and contributes to the rapid rise of obesity-related diseases. We have recently shown that<br> prenylcysteine oxidase 1 (PCYOX1) is a regulator of atherosclerosis-disease mechanisms, which<br> acts through mechanisms not exclusively related to its pro-oxidant activity. To address the role<br> of PCYOX1 in the adipogenic process, we extended our previous observations confirming that<br> Pcyox1//Apoe/ mice fed a high-fat diet for 8 or 12 weeks showed significantly lower body<br> weight, when compared to Pcyox1+/+/Apoe/ mice, due to an evident reduction in visceral adipose<br> content. We herein assessed the role of PCYOX1 in adipogenesis. Here, we found that PCYOX1 is<br> expressed in adipose tissue, and, independently from its pro-oxidant enzymatic activity, is critical for<br> adipogenesis. Pcyox1 gene silencing completely prevented the differentiation of 3T3-L1 preadipocytes,<br> by acting as an upstream regulator of several key players, such as FABP4, PPAR , C/EBP. Proteomic<br> analysis, performed by quantitative label-free mass spectrometry, further strengthened the role of<br> PCYOX1 in adipogenesis by expanding the list of its downstream targets. Finally, the absence of<br> Pcyox1 reduces the inflammatory markers in adipose tissue. These findings render PCYOX1 a novel<br> adipogenic factor with possible pathophysiological or therapeutic potential.</p><p>This work was supported by the Italian Ministry of Health, Italy, Ricerca Corrente 2021, Centro Cardiologico Monzino IRCCS</p&gt