STAT3 and 5 role in GC cytokine mediated C promoter epigenetic remodeling.

Abstract

(A) Schematic diagram of PROMO [59,60] predicted STAT binding sites on C promoter. (B-C) ChIP-qPCR analysis of STAT3 (B) or STAT5 (C) C promoter occupancy in GM12878 mock treated or treated with IL-15 or IL-21 for six days. (D-G) ChIP-qPCR analysis of Cp H3K27Ac (D), H2AK119Ub (E), H3K9me2 (F) and H3K9me3 (G) abundances in GM12878 expressing control versus STAT3/5A/5B targeting sgRNA, mock treated or treated with IL-15 or IL-21 for six days. All ChIP results are presented as % input mean ± SD from n = 3 replicates. Relative fold changes to Mock treated cells are labeled. *p p p < 0.001.</p