睾丸特异性丝氨酸蛋白激酶 （TSSK） 功能丧失引发入侵性飞蛾的男性不育症

Abstract

we identified that the testis-specific serine/threonine kinases (TSSKs) family is uniquely expressed in the testes of Cydia pomonella, a prominent global invasive species. We further generated male moths with disrupted the expression of TSSKs and those with TSSKs completely knocked out using RNA interference and CRISPR/Cas 9 genetic editing techniques, resulting in significant disruptions in spermiogenesis, decreased sperm motility, and hindered development of fertilized oocytes. Further explorations into the underlying post-transcriptional regulatory mechanisms have revealed the involvement of lnc117962 as a competing endogenous RNA (ceRNA) for miR-3960, thereby regulating TSSKs. Notably, orchard trials have demonstrated that the release of male strains can effectively suppress population growth. </p