Fabrication of Bovine Serum Albumin Nanoparticles Self-assembled Coacervation Method for Drug Delivery Systems

Abstract

Bovine Serum Albumin (BSA) nanoparticles have been produced in the particle size between 112 to 468 (nm). Nanoparticles were purified with 48,800 g centrifuge following by dialysis, micro and ultra filtration. Photon Correlation Spectroscopy (PCS) and Scanning Electron Microscopy (SEM) were used to characterize the nanoparticles. Photographs obtained from SEM image shown nanoparticles have semi-spherical shape. However the influence of several parameters such as the rate of addition desolvating agent,the concentration of BSA, the gluttaraldehyde concentration, agitation speed and BSA/Ethanol volumetric ratio have been investigated while the temperature kept in 14°C.The mean nanoparticle size diameter has not been relatively changed by variation of solvent adding rate and cross linker concentration and in contrast mainly depended on the volumetric ratio of BSA/ Ethanol, BSA concentration and agitation speed