Study on heat shock protein and thermotolerant mechanism of S. cerevisiae(耐热酵母菌株HU-TY-1的耐热机理初探)

Authors
Publication date
1 November 2001
Publisher
'Zhejiang University Press'

Abstract

以酿酒酵母耐热菌株HU-TY-1和亲株LK为材料，通过SDS-聚丙烯酰胺凝胶电泳等技术，对它们在热休克应答前后的耐热性及蛋白图谱进行了分析，发现两个菌株经温和高温(37 ℃)诱导后可大幅提高对致死温度(53 ℃)的耐受力，同时伴随着分子量为72 kD和84 kD的热击蛋白(HSP)的合成，其中耐热株HU-TY-1在常态时就有这两种热击蛋白的少量表达.由此表明：耐热酵母菌株HU-TY-1的高耐热性与HSP72和HSP84的组成性合成密切相关

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