Cellular membranes are complex systems that consist of hundreds of different lipid species. Their investigation often relies on simple bilayer models including few synthetic lipid species. Glycerophospholipids (GPLs) extracted from cells are a valuable resource to produce advanced models of biological membranes. Here, we present the optimisation of a method previously reported by our team for the extraction and purification of various GPL mixtures from Pichia pastoris. The implementation of an additional purification step by High Performance Liquid Chromatography-Evaporative Light Scattering Detector (HPLC-ELSD) enabled for a better separation of the GPL mixtures from the neutral lipid fraction that includes sterols, and also allowed for the GPLs to be purified according to their different polar headgroups. Pure GPL mixtures at significantly high yields were produced through this approach. For this study, we utilised phoshatidylcholine (PC), phosphatidylserine (PS) and phosphatidylglycerol (PG) mixtures. These exhibit a single composition of the polar head, i.e., PC, PS or PG, but contain several molecular species consisting of acyl chains of varying length and unsaturation, which were determined by Gas Chromatography (GC). The lipid mixtures were produced both in their hydrogenous (H) and deuterated (D) versions and were used to form lipid bilayers both on solid substrates and as vesicles in solution. The supported lipid bilayers were characterised by quartz crystal microbalance with dissipation monitoring (QCM-D) and neutron reflectometry (NR), whereas the vesicles by small angle X-ray (SAXS) and neutron scattering (SANS). Our results show that despite differences in the acyl chain composition, the hydrogenous and deuterated extracts produced bilayers with very comparable structures, which makes them valuable to design experiments involving selective deuteration with techniques such as NMR, neutron scattering or infrared spectroscopy.We are grateful to the ILL and the ESRF for awarding beamtimes (DOI: 105291/ILL-DATA.EASY-975) and (DOI: https://doi.org/10.15151/ESRF-DC-1026409781) respectively. Lipids were produced in the L-Lab (www.ill.eu/L-Lab) facility within the PSCM initiative at the ILL from biomass prepared in the D-Lab. We are grateful to Hanna Wacklin-Knecht (ESS) for useful discussions. This project received funding from the European Union's Horizon 2020 research and innovation program under grant agreement N 654000 (SINE2020) and from the League of advanced European Neutron Sources (LENS). CB, YYB and the GEMELI Lipidomic platform were supported by Agence Nationale de la Recherche, France (Project ApicoLipiAdapt grant ANR-21-CE44-0010), the Fondation pour la Recherche Médicale (FRM EQU202103012700), Laboratoire d'Excellence Parafrap, France (grant ANR-11-LABX-0024), LIA-IRP CNRS Program (Apicolipid project), the Université Grenoble Alpes (IDEX ISP Apicolipid), Indo-French Collaborative Research Program Grant CEFIPRA (Project 6003-1), and Région Auvergne Rhone-Alpes for the lipidomics analyses platform (Grant IRICE Project GEMELI). A.M. acknowledges the financial support from MICINN under grant PID2021-129054NA-I00.Peer reviewe
