The Effect Of Enteral Glutamine To Increase The Macrophage Count In Full-Thickness Burns Infected With Acinetobacter Baumannii Bacteria In White Rats (Rattus norvegicus)

Abstract

In this study, we explore the impact of hypermetabolic conditions on the immune function of the body, with a particular focus on the role of glutamine, an amino acid involved in protein synthesis and the regulation of metabolic processes within the immune system. Macrophages and monocytes play a crucial role in responding to A.Baumanii infection, being the first line of defense. Our investigation aims to analyze the augmentation of macrophage cell count activity against A. Baumanii through the administration of glutamine. Using an experimental study design, 45 rats were randomly assigned to three groups: a control group, a Glutamine treatment group, and a Glutamine against A. Baumanii group. The rats were evaluated on days 1, 5, and 7, with enteral administration of glutamine at a dose of 1 g/kg body weight/day. Specimens were taken from the peritoneum tissue, and anatomical pathology preparations were conducted to calculate the number of macrophage cells. Data collected were then input into a table and processed using SPSS 26 for Windows. Results revealed a significant increase in macrophage cell count on day 1 in the Glutamine against A.Baumanii group compared to the control group (p < 0.05). On day 5, the macrophage cell count in both the Glutamine group and the Glutamine against A.Baumanii group was higher than in the control group, with significant differences observed between groups (p < 0.05). On day 7, a significant difference (p < 0.05) in the number of macrophage cells was noted between the Glutamine groups. In conclusion, enteral glutamine feeding led to a notable increase in the number of macrophage cells, indicating a positive impact on the immune system. This rise in macrophages correlates with enhanced phagocytic activity against A.Baumanii infection