The dataset contains growth curves of E. coli RJA002 in various growth media in the presence of beta-lactam antibiotics. These growth curves were used to determine the degradation rate of these antibiotics in a range of experimental conditions. The degradation rate of mecillinam in MOPSgluRDM was also measured using Raman spectroscopy the data for which are also provided.Brouwers, Rebecca; Vass, Hugh; Allen, Rosalind J. (2020). Degradation of beta-lactams in bacterial growth media, [dataset]. University of Edinburgh. School of Physics and Astronomy. Institute for Condensed Matter and Complex Systems. https://doi.org/10.7488/ds/2824