Photodynamic therapy for ex vivo purging of malignant cells from ovarian cortex before autotransplantation

Abstract

In 2020, over 33,000 estimated cases of new leukemia instances in girls aged between 0 and 19, highlighting the pressing need for viable fertility preservation solutions. Ovarian tissue cryopreservation is the most suitable option to preserve fertility in prepubertal girls diagnosed with cancer. However, ovarian tissue transplantation in leukemia patients following cancer remission is not advisable due to the high risk of ovarian metastasis and, consequently, the potential reintroduction of malignant cells that might be present within the cryopreserved ovarian tissue. To address this critical concern and enable the secure transplantation of ovarian tissue from leukemia patients to restore their fertility, we aim to develop innovative photodynamic therapy strategies for the ex vivo eradication of leukemia cells. By employing a multifaceted approach, we have conceptualized and refined methodologies to formulate the most effective ex vivo purging protocol, targeting acute myelogenous leukemia cells within ovarian tissue fragments. These strategies have been carefully designed to safeguard the survival and development of ovarian follicles, so they can be deemed a potential fertility restoration alternative. By combining photodynamic therapy with nanotechnology, this research could greatly improve fertility preservation for leukemia patients, helping them achieve their dream of becoming parents.(BIFA - Sciences biomédicales et pharmaceutiques) -- UCL, 202