Dynamic analysis of the immunological response of Balb/c mice with experimental breast cancer submitted to immunotherapy treatment of dendritic cell/ Análise dinâmica da resposta imunológica de camundongos Balb/c com câncer de mama experimental submetido a imunoterapia de células dendríticas

Abstract

INTRODUCTION: Cancer is a complex disease because it is capable of inhibiting the immune response through tumor escape mechanisms. OBJECTIVE: analyze the dynamics of the immune system during cancer treatment with dendritic cell immunotherapy. We evaluated the presence of tumor infiltrate of CD8 T lymphocytes, transcription factors of CD4 T lymphocytes in the spleen of these animals, the development of tumor volume and the behavior of coestimulatory molecules.  METHODS: 70 female Balb/c mice were divided into experimental and control groups, they were evaluated on the 7th e 14th days after tumor challenge and dendritic cell immunotherapy. RESULTS: Molecules such as T-bet, showed an increased expression in treated tumor group. Our results also demonstrated the higher MFI of CD8 + T lymphocytes infiltrate in the treated groups. Thus, there is a greater MFI of protumoral co-stimulatory molecules such as CTLA4 in the untreated groups. CONCLUSION: the immune system is able to modulate an immune response against tumor within 14 days if the organism is being treated with dendritic cell immunotherapy