Western-style diet changes murinometric and metabolic parameters of rat offspring in time-specific windows / A dieta do estilo ocidental altera parâmetros murinométricos e metabólicos da própria de rat em janelas específicas

Abstract

The present study evaluated the effects of the western-style diet in the time-specific windows of rodents. Male Wistar rats were divided into three groups fed on a Control diet during all experimental period (CC); a western-style diet after lactation (CW); and a westernized diet throughout entire experimental period (WW). Body weight, food and energy intake, abdominal fat, glucose tolerance, biochemical parameters and serum hormones were evaluated. Higher body weight (CC=408.6±11.3g; CW=474.1±9.2g; WW=475.8±16.3g, p<0.001), increased abdominal fat (CC=12.6±0.8g; CW=34.1± 2.3g; WW=33.2± 2.4g, p<0.001), reduced food intake (CC=26.3±0.8g; CW=17.0±0.6; WW=18.4±0.9g, p<0.001), and similar energetic intake were found among the groups fed on the western-style diet regardless to the exposure time. Also, the area under the curves of glucose tolerance test, glucose, triacylglycerol, VLDL-c and total cholesterol blood levels were higher in the western-style diet groups compared to the control groups (p<0.01). However, levels of hormones were different according to specific windows of dietary exposure. The CW group showed higher corticosterone (CC=263.4± 109.1;CW= 339.9±16.6; WW=305.5±16.2ng/mL, p<0.001) and leptin levels (CC=8.2 0.7;CW=14.5 1.4;WW=9.1 0.7ng/mL p<0.001); and the WW group showed raised insulin levels (CC=6.7±0.3;CW=8.0±0.3;WW=10.5±0.8ng/mL, p<0.001). Then, the western-style diet enhanced signals of metabolic imbalance in both groups, but the outcomes varied according to the windows of dietary exposure