This work evaluates the possibility to substitute external soya bean, a high risk GMO alimentary source, with other legumes produced on farm, such as sweet lupin, protein pea and field bean, as alternative protein source in the formulation of diet in organic dairy cattle nutrition. In 2005/2007 periods both the field and feeding trials were carried out in an organic dairy farm in Tuscany. The performances of grain legumes crops were evaluated in terms of grain yield and quality of grains. The alimentary experiment was carried out on dairy cattle fed with two diets: A with extruded soya bean and B with bitter lupin + field bean + high protein pea. In the field trial the Italian sweet lupin varieties (Multitalia) were the most interesting for CP production and pea the best for yield. The feeding trial provided that the protein content was higher for the A diet (with soya bean) while fat, somatic cells and urea content did not differ

Casini, M.

Giorgetti, Alessandro

Lorenzini, Giangiacomo

Lotti, Claudia

Martini, Andrea

Migliorini, Paola

Riccio, Federica

Rosi Bellière, Samanta

Squilloni, Stefano

Organic Eprints

16th IFOAM Organic World Congress, Modena, Italy, June 16-20, 2008Archived at http://orgprints.org/11957Production of grain legume crops alternative to soya bean and their use in organic dairy productionMartini, A.​[1]​, Migliorini P. ​[2]​, Lorenzini, G.1, Lotti, C.1, Rosi Bellière S. 2, Squilloni, S.1, Riccio, F.1 Giorgetti, A.1, Casini M.3Key words: high protein pea, field bean, lupin, dairy cattleAbstractThis work evaluates the possibility to substitute external soya bean, a high risk GMO alimentary source, with other legumes produced on farm, such as sweet lupin, protein pea and field bean, as alternative protein source in the formulation of diet in organic dairy cattle nutrition. In 2005/2007 periods both the field and feeding trials were carried out in an organic dairy farm in Tuscany. The performances of grain legumes crops were evaluated in terms of grain yield and quality of grains. The alimentary experiment was carried out on dairy cattle fed with two diets: A with extruded soya bean and B with bitter lupin + field bean + high protein pea. In the field trial the Italian sweet lupin varieties (Multitalia) were the most interesting for CP production and pea the best for yield. The feeding trial provided that the protein content was higher for the A diet (with soya bean) while fat, somatic cells and urea content did not differ. IntroductionGrain legumes crops represent a great resource in organic agriculture both to satisfy the nutritional content of organic livestock feeding and to maintain soil fertility. The commercial availability of organic grain legume is decreasing, the costs are high and the GMO contamination risk is particularly high for soya bean, used to achieve the high protein values required by the animals. So, the cultivation of grain legumes such as sweet lupin (Lupinus albus), field bean (Vicia faba var. minor), high protein pea (Pisum sativum) on farm could solve the problem and improve the sustainability of the farm. In particular lupin appears more interesting and promising. It has a DM yield in grain of 1-4 t/ha with a crude protein (CP) and oil content of 30 - 35 % and 10%, respectively (on DM). On contrast soy bean, a high risk GMO supplement, has a DM yield in grain of 2784 kg/ha and 40 - 41% of CP (on DM). Although sweet lupin is widely used in Northern Europe and other large areas of the world, in Italy it is not anymore widely cultivated and only one registered variety (Multitalia) is available. In this work we evaluate the substitution of soya bean with lupin to dairy cattle diet in terms of milk production.Materials and methodsThe performances of grain legumes crops (sweet lupin, field bean and high protein pea) were evaluated in an organic dairy farm of Tuscany in 2005-06 (Migliorini et al., 08) and 2006-07 in terms of grain yield, competitive ability against weeds and quality of grains. The feeding trial was carried out on 36 dairy cattle of the Italian Holstein breed from the same dairy farm in Tuscany, divided in two groups, and fed with diet A, containing soya bean and diet B, containing lupin, from June 2006 to March 2007. To avoid the influence of age, season and ration, each group of 18 animals was composed with the same number of primiparous (9) and pluriparous (9), 6 in the 1st 100 days of lactation, 6 the 2nd (100-200 days) and 6 in the last part (>200 days). The two diets (table 1) were conform to the Reg CE 2092/91 for concentrate/forage ratio and they satisfied the energetic and protein needs of 600 kg milking cows with 32,5 kg/day milk production at 4% of fat (INRA, 1988). Feeds were analysed in order to determine dry matter (DM), crude protein (CP), fat, crude fiber (CF), ash according with AOAC methodology (AOAC, 1990) and fibrous fraction (NDF, ADF, ADL) according with Van Soest et al. (1991). Unfortunately, we were limited by commercial reasons to the use of bitter lupin and the one produced in the farm was not yet available. In a previous trial, in order to investigate alkaloids and anti-nutritional factors contained in the bitter lupin (Singh et al., 1994; El-Adawy et al., 2001), we compared two different diets (with and without bitter lupin) to evaluate the apparent digestibility (Lorenzini et al., 2007). Moreover, in order to eliminate the bitter flavour of the lupin bean that cattle seemed not to like, it was necessary to crush and mix the lupin with field bean and protein pea, to make it more appealing to the animals.Tab. 1: Characteristics of the two diets provided to two groups of milking cow. Components	Dry matter kg	Crude protein kg	UFL	Diet A	Diet B	Diet A	Diet B	Diet A	Diet BAlfalfa hay	1.7	1.7	0.2	0.2	1.1	1.1May hay	0.9	0.9	0.1	0.1	0.5	0.5Maize silage	7.2	7.2	0.6	0.6	6.1	6.1Alfalfa silage	2.1	2.1	0.3	0.3	1.5	1.5Corncob silage	3.4	3.4	0.3	0.3	3.1	3.1Extruded soya bean	0.9	-	0.4	-	1.1	Barley	2.7	2.7	0.3	0.3	3.1	3.1Bitter lupin	-	0.8	-	0.3		0.9Field bean + protein pea	2.2	2.9	0.6	0.8	2.3	2.9Total	21.1	21.7	2.8	2.9	18.8	19.2Notes	For. 72%/ Conc. 28%	For. 71%/ Conc. 29%	13.3% DM	13.4% DM	0.9 UFL/kg DM	0.9 UFL/kg DMAnalysis of variance (ANOVA) was applied to milk production using SAS statistical procedures considering as fixed factor diet regime (diet A and diet B) and lactation phase.Results and DiscussionThe quantity and quality parameters of grain legumes cultivated on farm in 2005-06 are shown in table 2. Considering only the crude protein content, sweet lupin var. Multitalia is the best variety producing 1.607 kg/ha of protein, almost double the field bean var. Vesuvio (CP 819 kg/ha), the less productive one. The protein pea crop varieties, although the CP content is not very high, are very interesting for the production of total CP, due to the good yields. The field bean produced the lower CP total quantity, due to lower yield, compared to others grain legumes. The varieties sown in spring (Pea Hardy and Lupin Luxe) didn’t manage to mature properly before the warmth and, except for Lupin Multitalia, their yields were zero.Tab. 2: Characteristics of the grain legumes produced in a Tuscan organic farm in 2006. SpeciesVariety	GYDM (t/ha)	DM%	CP% DM	FAT% DM	CF% DM	Ash% DM	NDF% DM	ADF% DM	ADL% DM	CP (kg/ha)F. Vesuv.	3.16	93.65	25.93	0.88	13.77	3.82	30.37	19.86	5.01	819F. Chiaro	3.49	93.93	27.66	0.76	9.37	3.83	34.58	14.12	2.87	965P. Class.	5.36	93.75	21.14	1.25	5.16	3.09	30.39	9.73	0.50	1133P. Hardy a	6.03	93.68	20.32	1.29	1.45	3.10	31.03	11.32	0.10	1225P. Ideal	5.15	94.53	23.60	1.19	8.01	3.17	30.48	11.82	1.11	1215P. Hardy s	0.00	93.33	22.41	1.08	10.34	3.62	32.73	18.51	2.03	0L. Multi.	4.50	95.05	35.72	3.96	15.61	8.68	33.77	26.34	4.42	1607L. Luxe	0.00	95.05	36.36	5.60	16.66	4.58	27.16	23.23	4.92	0Tab. 3: Result of milk productions (diet A, with soya bean and B, with lupin)DFR = 306		Treatment	Lact. per.	Treatment x Lactation period		Mean	Sig.	Sig.	0-100 days	100-200 days	>200 days	Sig.Milk kg	Diet A	32.5	***	***	36.7	33.2	30.0	ns	Diet B	27.8			30.0	28.5	24.6	Fat %	Diet A	4.1	ns	ns	4.1	4.1	4.0	ns	Diet B	4.1			4.3	3.9	4.2	Protein%	Diet A	3.3	**	***	3.0	3.2	3.4	ns	Diet B	3.1			2.9	3.1	3.4	SCCx103/ml	Diet A	361	ns	ns	503.0	437.6	251.9	ns	Diet B	267.1			266.5	196.7	362.6	Urea g/100 ml	Diet A	0.026	ns	ns	0.027	0.025	0.026	ns	Diet B	0.026			0.027	0.026	0.028	* significant for P<0.05 **significant for P<0.01 *** significant for P<0.01The results of milk production of the two group fed with different diet are shown in table 3. Although the quantity and quality of milk of the B cow group, fed with bitter lupin, is good, is not the same of the A cow group, fed with soya bean. In particular the alimentary treatment and the lactation period (0-100 days, 100-200 days, >200 days) had a positive influence on the diet A for milk production (+4,7 kg/day) and protein content (+0,2%), while no influence on fat, somatic cells and urea content was observed. Although the interaction of the two parameters did not show significant differences, we reported the mean value in the table 3 to get an idea of the lactation curve of the two experimental groups. The bitter lupin has never influenced the urea contents in the milk, always at physiological levels in both the experimental groups. It means that it didn’t negatively influence the protein metabolism.ConclusionsBecause the climate (often too dry, too hot and too cold) and soil characteristics (rocky soils), the Mediterranean area normally offers poor pastures and scarce possibilities to produce a sufficient amount of vegetal protein sources to feed dairy cattle (Boyazoglu and Morand, 2000). But, alternative of buying soya bean is feasible in many countries of this area, like in the Central Italy. In fact the production on farm level of field bean, protein pea and sweet lupine provide considerable contributions of nutrient for cattle. In particular, sweet lupine var. Multitalia produced the highest total protein content, while the other non-Italian varieties failed. Protein pea is very interesting for its higher grain yield while field beans may suffer under climatic condition resulting in lower yields. Anyway more research work is needed on the use of local varieties. The introduction of lupine as alternative to soy bean in the formulation of diet in organic dairy cattle nutrition is interesting also in case of bitter lupine. In fact, when mixed with field bean and protein pea, to make it more palatable, the quantity and quality productive levels are slightly lower in comparison with soy bean diet.AcknowledgmentsThe work was partly funded by the interregional project EQUIZOOBIO. Special thanks are due to Coop. Emilio Sereni for hosting the experimental trial in its organic farms.References Boyazoglu J., Morand-Feher P. (2001) Mediterranean dairy sheep and goat products and their quality. A critical review. Small Ruminant Research 40, 1-11El-Adawy, T.A., Rahma, E.H., El-Bedawey, A.A., Gafar, A.F. (2001): Nutritional potential and functional properties of sweet and bitter lupin seed protein isolates. Food Chem. 74:455–462.INRA. (1988):Tables de l’alimentation des bovins ovins & caprins. INRA editions, Paris, France.  Lorenzini G, Martini A., Lotti C., Casini M., Gemini S., Riccio F., Squilloni S., Rovida A., Tocci R. (2007): Influence of bitter lupin on ingestion and digestibility in organic dairy cattle soya free diets. IJAS vol. 6 supp.1 pp. 657-659. Migliorini P., Tavoletti S., Moschini V. & Iommarini  L. (2008) Performance of grain legume crops in organic farms of central Italy. International ISOFAR Conference. Modena 2008. In pressSingh, C. K., Robinson, P. H., McNiven, M. A. (1995): Evaluation  of raw and roasted lupin seeds as protein supplements for lactating cows. Animal Feed Science and Technology. 52:63-76. Van Soest, P.J., Robertson, J.B., Lewis, B.A. (1991):  Methods for dietary fiber, neutral detergent fiber, and non starch polysaccharides in relation to animal nutrition. J. Dairy Sci. 74, 3583 – 3597. ^1	  Department of  Scienze Zootecniche University of Florence; via delle Cascine 5,  50144 Firenze – Italy, E-Mail andrea.martini@unifi.it, internet  www.zoot.unifi.it^2	  Department of Agronomy and Land Management (DISAT), University of Florence Piazzale delle Cascine 18, 50144 Florence, Italy, E-Mail paola.migliorini@unifi.it, Internet www.unifi.it/disat3 Cooperativa Agricola Emilio Sereni, via La Brocchi, 27, 50032 Borgo S. Lorenzo (FI), E-Mail coop.agricolaemilioseren@tin.it

Production of grain legume crops alternative to soya bean and their use in organic dairy production

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Production of grain legume crops alternative to soya bean and their use in organic dairy production

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