The aim of this study was to detect the contamination of Geobacillus stearothermophilus in crab meat canned product by using bacteriological test and Polymerase Chain Reaction (PCR). The sample used in this study was commercial crab meat product in the laboratory of Tropical Disease Diagnostic Center, AirIangga University. The isolation and identification of Geobacillus stearothermophilus used in the study were include bacteriological method and PCR test. The characteristics of this bacteria were identified as Geobacillus stearothermophilus by their morphological characteristics, Grram staining, and biochemical test (H2S, glucose, xylose, sucrose, lactose, mannitol, indole and Voges-Proskauer) by using Oxoid Microbact™ Kit (l2A + 12B) within 75% biochemical properties similarity to Geobacillus stearothermophilus, while it's lipase gene was amplified in a band of 727 bp
