The Difference of Plasma Prothrombin Time Value in the
Production of Adsorbed Plasma Using Various Concentrations
of Barium Sulphate in Dl: Soetomo General Hospital,
Surabaya, Indonesia
Detection ofa factor deficiency in the case of coagulation prolongation is veIY imp01tant. in this case a factor
deficient plasma is required. This research provides a simpler method to produce factor deficient plasma.
using barium sulphate as an adsorbent with Prothrombin Time (PD of more than 60 seconds as a target.
The aim of this study is to analyze the difference ofPT value in various concentrations of barium sulphate.
Pooled nonnal plasma (Pl'\lP) was made fi'om citrated blood and basic PT value was measured. Nine plain
tubes were prepared with 1 mL of Pi\lP for each tube. Barium sulphate 200-1000 mg/ml plasma was then
added to each tube. Finally. PT value of each tube was measured. The presence of barium sulphate <800 mg/
ml plasma made no significant changes in PT. Conversely. the presence of2':800 mglml plasma increased
PT more than 5 times compared to nonnal PT. Adsorbed plasma lack vitamin K dependent factors because
barium sulphate binds to a -polypeptide. and removes all vitamin K dependent factors. The barium sulphate
concentration used to make adsorbed plasma in this research was different with previous research. It was
found that barium sulphate with 2':800 mglml plasma can significantly change the PT value. and effectively
reach the PT target of adsorbed plasma as a basic reagent for substitution study. In conclusion. different
concentrations of barium sulphate used resulted in different PT values. Optimal concentration of barium
sulphate to make adsorbed plasma was 2':800 mg/ml plasma
