One of the reproductive aspects of development step that is considered as the
solution of this issue is seaweed sporulation technique through which is induced through
salinity and temperature shock. This study aims to determine the effect of combination and
interaction of salinity and temperature shock on the release of K. alvarezii spores in order to
produce superior seeds. This research was conducted using Complete Randomized Design
Factorial which consists of nine combinations of treatments and three replications. The used
treatment in this study is the combination of different environmental factors such as salinity
shock and temperature shock. The data were analyzed using ANOVA (Analysis of Variance)
followed by Duncan Multiple Range Test. The results showed that salinity (31 ppt, 33 ppt, and
35 ppt) and temperature (30˚C, 32˚C, and 34˚C). shock affected the osmoregulation system
and the release of K. alvarezii spores. The salinity shock and temperature shock had interaction
with K. alvarezii spore release on the sixth and seventh day with the best treatment at 32°C
temperature and 31 ppt salinity and released 5413 cells/ml spores on the seventh day
