Perbedaan Komponen Protein Sel Utuh Bakteri Salmonella typhirnurium Yang Di Isolasi Dari Penderita Diare Dan Daging Ayam Ras: Penelitian Eksplorasi Laboratorik
Salmonellosis is recognized to be a serious public health disease problem, affecting more people and animal than any disease problem. Salmonella is capable of causing a variety of disease syndromes : enteric fever, bacteremia, enterocolitis and focal infention. The virulence properties in S. typhimurium are often mediated by genes contained within pathogenicity islands, i.e., large, unstable segments of the chromosome. Virulence plasmid and Inv locus are also important in virulence of bacteria Environment stress affected S. typhimurium protein components. There is an accumulation of glutamate in response to osmotic stress and production of specific outer membrane protein were induced by acid adaption. Four S.typhimurium isolates are identical each other by biochemistry and serological tests, two isolates came from diarrheal stool children (H-1 and H-2) and two remaining came from chicken meat (M-1 and DS-9), respectively. Whole cells protein analysis were done by SDS-PAGE with 7,5 % acrylamide gel concentration. The result showed that S. typhimurium H-1 isolate (from diarrheal stool children) had components protein differences with another isolates (H-2., from diarrheal stool sample, M-1 and DS-9, both came from chicken meat)
