The objective of this study was to determine the variability in N use efficiency among field-grown timothy (Phelum pratense L.) populations. Shoot biomass and N uptake were measured at the end of the spring growth cycle on six timothy populations fertilized with three N rates at two sites in Eastern Canada. The variability in shoot biomass among populations was similar under limiting and nonlimiting N conditions. The ranking of the populations, however, differed under limiting and non-limiting N conditions, and also between the two sites under limiting N conditions. The differences in shoot biomass among populations under highly N deficient conditions were more related to N conversion efficiency than N uptake efficiency. These preliminary results indicate significant interactions between the N nutrition status and timothy populations, and the importance of N conversion efficiency under highly N deficient conditions