The pressing need to mitigate environmental concerns has driven research into sustainable energy
and chemical production methods that reduce carbon emissions. Gas fermentation offers a promising
avenue for low-carbon fuel and chemical synthesis. Acetobacterium wieringae, particularly strain A.
wieringae JM, has emerged as an attractive host for gas-based biorefineries due to its unique
abilities, including growth in diverse gas compositions and pH ranges, and efficient growth on
carbon monoxide without co-substrates.
This study focuses on enhancing the potential of A. wieringae for acetone production through
genetic modification. A transformation protocol was developed, and the acetone production operon
from Clostridium acetobutylicum was introduced. Novel promoters were explored to widen gene
expression possibilities in A. wieringae. The stability of the plasmid backbone pMTL83151 carrying
replicon pCB102 was assessed. Additionally, the tolerance of A. wieringae to gas synthesis derived
from biogenic residue gasification was evaluated for potential industrial application.
Gas composition significantly influenced acetone production by A. wieringae, with distinct
physiological effects observed between strain A. wieringae DSM 1911 and
A. wieringae JM. Four constitutive promoters from A. wieringae JM and four from C.
autoethanogenum were successfully expressed, exhibiting stronger activity than the reference Pthl
promoter from C. acetobutylicum. Notably, A. wieringae JM demonstrated robust growth in synthesis
gas from biomass gasification, though with physiological variations.
This study unveils the intricate relationship between gas composition, physiological attributes, and
acetone production in A. wieringae. The expanded promoter repertoire enhances genetic
manipulation potential, propelling the strain's capacity for versatile gene expression. Moreover, the
resilience of A. wieringae JM to gasification-derived gas synthesis highlights its viability for
industrial implementation. These findings contribute to advancing the development of gas-based
biorefineries, paving the way for sustainable chemical production with reduced environmental impact.info:eu-repo/semantics/publishedVersio
