REGULATION OF SOX 4 AND SOX 11-MEDIATED GENE TRANSCRIPTION BY MIR-30-BASED RNAi

Abstract

This projects goal was to use a plasmid previously cloned in our laboratory encoding miR30 to determine its effectiveness to knock down the expression of Sox4 and Sox11, two genes closely associated with tumor initiation and progress. Western blots and luciferase reporter assays showed that miR30-based shRNAs resulted in strong knock-down of Sox4 and Sox11 expression and a decrease in their induction of Sox-mediated gene transcription in 3T3 cells. Our results suggest that miR30-based shRNAs might be used as an effective cellular tool to investigate the role of Sox4/11 in tumorigenesis