Isolation And Identification Of Bacteria From Solid Waste Deposit Sites For The Degradation Of Pollutants

Abstract

In and around the Treatment Storage and Disposal Facility (TSDF), Duindigal, Ramky site, Hyderabad, Telangana, ten samples were collected. To assess the ability of the bacterial isolates to remove contaminants, the following parameters were chosen for this study: pH, TDS, BOD, COD, TN, and TP. Two of the ten isolates showed promising degradation capabilities. The 16S rRNA gene sequence was used to apply genotypic techniques to the two strains that performed the best. Alcaligenes faecalis and Brevibacterium iodinum are the two bacterial strains that we found using NCBI BLAST analysis. To optimize the growth rate of the isolates, some parameters like pH, temperature, incubation time and peptone concentrations were optimized and got enhanced results. These findings have expanded the possibility of identifying industrially significant bacteria from solid waste disposal sites, and these isolates may be an essential source for the identification of enzymes with practical applications in industry