Light is a fundamental environmental factor that profoundly influences various physiological and behavioral processes in animals, including humans. This study aimed to investigate impact of light patterns on psychophysical behavior (such as depression-like behavior, anxiety-like behavior, social interaction), reproductive hormones (including testosterone, LH, FSH, and GnRH) in Wister rats. A total of twenty-eight male Wistar rats were grouped into four (4) groups: Group I: Control group which received normal light, Group II: Rats kept in total darkness (no light source), Group III: Rats expose to Tonic/constant light, Group IV: Rhythmic light (off and on flickering light). Data were analyzed using GraphPad Prism v9.0 version.Results demonstrated that exposure to rhythmic light, total darkness, and bright light induced depression-like behavior, with rhythmic light having the most pronounced effect. Anxiety-like behavior was heightened in rats exposed to rhythmic light, aligning with disrupted light-dark cycles inducing anxiety-like symptoms. Social interaction was negatively influenced by total darkness and bright light, while rhythmic light promoted positive social behavior. Reproductive hormone levels, including testosterone, LH, FSH, and GnRH, were significantly impacted by light patterns. Bright light exposure was associated with increased FSH levels, while rhythmic light suppressed FSH production. However, GnRH levels were elevated by bright light and reduced by other light patterns. Additionally, exposure to total darkness led to increased body weight in male rats, whereas rhythmic light was linked to reduced weight gain. The study underscores the intricate relationship between light patterns and physiological responses, contributing to a nuanced understanding of how light exposure influences behavior and hormonal regulation.
 
