Objective: In recent years, in vitro maturation (IVM) has become the focus of fertility maintenance, and infertilitytreatment. The aim of this study is development of oocytes during folliculogenesis and oogenesis is greatly influencedby the presence of BMP-7, BMP-15, and GDF-9 genes, which are present in exosomes generated from bone marrowstem cells.Materials and Methods: In the experimental study, we investigated how exosomes obtained from bone marrow stemcells affected development and expansion of ovarian granulosa cells (GCs) in NMRI mice. In this in vitro experiment,bone marrow stem cells were isolated from mice’s bone marrow, and after identification, exosomes were recovered.Exosome doses of 100, 50, and 25 μg/ml were applied to GCs before using MTT assay to measure survival rates andquantitative reverse-transcription polymerase chain reaction (PCR) to measure expression of the BMP-7, BMP-15, andGDF-9 genes.Results: The results showed that the GCs treated with exosomes concentrations of 25, 50, and 100 μg/ml significantlyincreased bioavailability, growth and proliferation and it also increased expression level of BMP-7, BMP-15 and GDF-9genes compared to the controls.Conclusion: Findings of this study indicated that exosomes derived from bone marrow stem cells improved growthof GCs in NMRI mice and they were a good candidate for further clinical studies to improve quality of the assistedreproductive techniques
