Comparative structural and functional characteristics of different forms of saccharomyces cerevisiae red pigment and its synthetic analogue

Abstract

Structural and functional characteristics of the yeast red pigment (product of polymerization of N'-flí-D-ri-bofuranosyl)-5-aminoimadazole), isolated from adel 1 mutant cells of Saccharomyces cerevisiae, its deribosy-lated derivatives (obtained by acid hydrolysis) and its synthetic pigment analogue (product of polymerization of N'-methyl-5-aminoimadazole in vitro) has been obtained. Products of in vitro polymerization were identified using mass spectrometry. The ability of these pigments to inhibit amyloid formation using insulin fibrils was compared. The entire compounds studied were able to interact with amyloids and inhibit their growth. Electron and atomic force microscopy revealed a common feature inherent in the insulin fibrils formed in presence of these compounds - they were merged into conglomerates that were more stable and resistant to the effects of ultrasound in comparison with insulin aggregates grown without pigments. We speculate that all these compounds can cause coalescence of fibrils, partially block their loose ends and, thereby, inhibit the attachment of new monomers to growing fibril