The development of simple, rapid, and sensitive methods for thiophenol detection is of great importance in various fields, including environmental monitoring and food quality. Here, we present a novel approach for the colorimetric detection of thiophenols using gold nanoparticles (AuNPs) as the sensing platform. AuNPs aggregate in the presence of thiophenols. The aggregation of the AuNPs causes a bathochromic shift which is qualitatively detected by color change and quantitatively measured using UV-VIS spectroscopy. This AuNP-based colorimetric assay offers a simple and effective method for the detection of thiophenols and has the potential to be further developed for other applications in environmental and biological sensing.Keywords: Gold nanoparticles, thiophenols, colorimetric assa