Measurement of relative fitness of dicarboximide-resistant botrytis cinerea in strawberry leaves

Abstract

Leaves of healthy strawberry plants were inoculated with either vinclozolin-sensitive or vinclozolin-resistant isolates of Botrytis cinerea at one of three concentrations: 5x104, 1x105, and 5x105 conidia/ml. One week following inoculation, representative leaves were harvested and cut into 9mm2 leaf pieces. Some pieces were immediately plated onto selective Botrytis medium while the others were stored for 7 or 21 days in an empty petri dishes to which silica gel had been added as a desiccant. Vinclozolin-resistant B. cinerea isolates established fewer infections in young, healthy strawberry leaves compared to vinclozolin-sensitive strains of the fungus. However, no observable differences in relative ability to survive over a month of desiccation were noted among resistant and sensitive strains

    Similar works