ABSTRACT: In September 2012, Pico Island was surveyed along an elevational transept starting on Manhenha, at 10 m a.s.l., and ending on the Pico Mountain caldera, at 2200 m a.s.l. to obtain a systematic inventory of the bryophytes living in natural environments. A total of twelve sites were examined, separated by 200 m elevation steps. At each site, two 10 m x 10 m plots were established within 10-15 m from each other, and within each plot, three 2 m x 2 m quadrats were randomly selected and thoroughly sampled for bryophytes using microplots of 10 cm x 5 cm, collected to paper bags. Six substrates were explored within each quadrat: rock, soil, humus, organic matter, tree bark and leaves/fronds; three replicates were made for each substrate, whenever available and colonized by bryophytes, totalling a maximum of 18 microplots per quadrat, 54 microplots per plot, 108 microplots per site and 1296 microplots on the 12 sites of Pico Island. It was possible to collect two thirds of the maximum expected number of microplots (N=878; 67,75%), resulting in 4896 specimens, the great majority of which (n=4869) were identified to the species/subspecies level. Overall, it was possible to identify 70 moss’ and 71 liverwort’ species and subspecies. The elevation levels both with a greater number of microplots and higher richness of species were those between 600-1000 m a.s.l., coinciding with the native forest plots. The study contributed to improve the knowledge of Azorean bryophyte diversity and distribution at both local and regional scales, including the recording of new taxa for Pico Island and the Azores.info:eu-repo/semantics/publishedVersio
