A study was conducted to isolate and examine the bacterial strains from litter areas. Two soil samples were collected from waste disposal sites, namely, industrial waste sites of Nilon’s pickle industry in Dalgaon and market waste sites of Balugaon vegetable market of Kharupetia in Darrang district (Assam). A total of nine bacterial strains were identified at industrial waste dumps, namely Leclercia adecarboxylata, two strains of Pseudomonas putidia, Ralstonia pickettii, three strains of Serratia marcescens and Stenotrophomonas maltophilia, and at market waste dumps, four out of five bacterial isolates were identified, namely two strains of Enterococcus cloacae, Bacillus megaterium and Bacillus coagulans using the Vitek-MS MALDI TOF system. For both soil samples, morphological, biochemical, physicochemical tests were carried out against all bacterial strains to identify a beneficial bacterial strain