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Haematococcus halophyllum chloroplast homologous recombination vacant carrier and application thereof
Authors
初金玲
崔玉琳
+3 more
林彬
焦绪栋
秦松
Publication date
23 August 2019
Publisher
Abstract
本发明涉及基因工程技术领域,具体涉及一种杜氏盐藻叶绿体同源重组空载体及其应用。载体包括启动子、终止子,所述重组空载体含SEQ ID NO : 1所示碱基序列的上游同源臂和SEQ ID NO : 2所示碱基序列的下游同源臂,同源臂之间插入与至少一个外源基因构成多顺反子结构的SEQ ID NO : 5所示的碱基序列。采用本发明的杜氏盐藻叶绿体稳定表达系统, 可实现多个外源基因在叶绿体中稳定表达
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Last time updated on 14/02/2024