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Method for detecting timing potential of sensitive film based on polymer obstruction
Authors
丁家旺
刘淑文
秦伟
Publication date
16 April 2021
Publisher
Abstract
本发明涉及基于阻碍的敏感膜电位分析技术,具体的说是一种基于聚合物阻碍敏感膜计时电位的检测方法。以聚合物膜离子选择性电极为工作电极,以待测目标物的核酸适体、DNA或抗原/抗体作为识别分子,通过在探针基体上负载或标记酶或其类似物分子作为识别探针,采用脉冲恒电流控制的计时电位技术对待检测液中目标物进行定量/定性的检测;本发明方法具有一定的通用性,可以通过改变识别分子的适体序列或采用DNA、抗原/抗体能够实现对不同目标物的检测。在检测过程中亦可以利用含有离子交换剂的敏感膜,通过测定电极的开路电位实现目标物的检测
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Last time updated on 14/02/2024