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Asparagus reagent level R - algae red protein efficient separation and purification method
Authors
刘冰
杜虹
+3 more
秦松
谷洋洋
陈洁辉
Publication date
29 March 2017
Publisher
Abstract
本发明属于海洋天然活性物质制备技术,具体的说是一种龙须菜试剂级R-型藻红蛋白的高效分离纯化方法。将粉碎的原料大型经济海洋红藻龙须菜加入其10-30体积的PBS缓冲液中浸泡,浸泡后以9000rpm/min-15000rpm/min离心20-30min,收集上清液进行浓缩;浓缩液经硫酸铵进行分级盐析,收集沉淀并用PBS缓冲液溶解,溶解后进行透析24--48小时;所得透析液经强阴离子Source-15Q层析柱纯化,以含NaCl的PBS缓冲液作为洗脱液,流速0.5ml/min, 收集第360min-510min的洗脱液,即为纯度大于5.0的R-型藻红蛋白溶液。采用本发明方法得到的蛋白回收率高,纯化效果好,易于放大,上样量大等特点实现了对藻红蛋白高效分离纯化
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Last time updated on 14/02/2024